GFP Green fluorescent protein Essay Example | Topics and Well Written Essays - 500 words

GFP young fluorescent protein - Essay ExampleMicroorganisms can be easily used for genetic tailoring by means of genetic tools to study the modifications in environmental conditions. Reporter genes are tagged with multicoloured fluorescent proteins which aid in identifying the chemical effects prevailing in the environment. The engineered microorganisms are then used for solely cell array set-up on silicon chips and diverse optic fibres to get bioavailability of pollutants which could be correlated with their pertain on life (Belkin, 2003).Reporter gene is verbalised when it is fused with a promoter target sequences. olibanum expression can be observed by the appearance of the reporter gene, in view of the event that reporter is tagged with the active gene of interest. The promoter is tagged with a marker gene much(prenominal) as GFP, a stable molecule used to identify the phenotype or the genotype of the transformed organism. freshness markers emit light making the selectio n process easy and simple moreover, they measure the cellular operation swiftly. FACS (fluorescence activated cell sorting) is performed with GFP, YFP and BFP i.e. green, yellow and blue fluorescent proteins respectively. The molecules do not require oxygen for fluorescence (Belkin, 2003).In transgenic organisms GFP molecule is integrated with active gene, expression of gene is observed through green glow down the stairs UV radiation. Transgenic organisms of various species such as E coli, C elegans, Drosophila were made to glow by inserting GFP reporter gene. Toxicity of GFP is not observed in both prokaryotic and eukaryotic organism, indicating the safety of the molecule. littler size of GFP makes it ideal for reporter gene or if GFP is tagged at the end of the active gene, it is expressed with the gene once the gene undergoes the process of transcription and translation, however, expression of GFP does not affect the protein conversely, the protein can be seen as green fluores cence indicating the